DNA

Part:BBa_K4121007:Design

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-04)


It is a promoter in Saccharomyces cerevisiae, and the expression intensity is relatively strong.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before using this part, give priority to whether it has the enzyme cleavage site required, if so, consider site-directed mutagenesis.


Source

EPD (Eukaryotic Promoter Database) website

References